Esports, particularly First-Person Shooter (FPS) games, rely heavily on one's ability to rapidly perceive and respond to visual targets, a skill known as target acquisition. Modern gaming monitors increasingly feature higher refresh rates (up to 360Hz). The current study examined whether FPS gamers can perceptually distinguish between different monitor refresh rates (60Hz, 144Hz, 360Hz) and whether these differences translate to performance improvements in target acquisition tasks. Gamers (N = 101) completed a custom FPS task across three refresh rate conditions.